IF 16+ 非小细胞肺癌的单细胞和空间转录组学分析

发布于：2025-07-29 ⋅ 阅读:(11) ⋅ 点赞:(0)

图片

摘   要

肺癌是第二常见的癌症，也是全球癌症相关死亡率的主要原因。肿瘤生态系统具有多种免疫细胞类型。特别是髓样细胞很普遍，在促进疾病方面发挥着公认的作用。在我们的研究中，我们通过单细胞和空间转录组学分析了25名腺癌和鳞状细胞癌初治患者的约90万个细胞。我们注意到抗炎巨噬细胞和NK细胞/T细胞之间存在负相关关系，并且肿瘤内NK细胞的细胞毒性降低。虽然我们在腺癌和鳞状细胞癌中观察到相似的细胞类型组成，但我们检测到各种免疫检查点抑制剂的共表达存在显著差异。此外，我们揭示了肿瘤中巨噬细胞转录“重编程”的证据，将其转移到胆固醇输出，并采用促进铁外流的胎儿样转录特征。我们的多组学资源提供了肿瘤相关巨噬细胞的高分辨率分子图谱，加深了我们对它们在肿瘤微环境中作用的理解。

数据收集

scRNA-seq (N=24 patients)

Visium (N=8 patients)

软件使用

单细胞分析软件包列表

软件包名称

主要应用领域

官方链接
CellRanger

单细胞RNA测序原始数据处理

10x Genomics
Harmony

多数据集批次校正与整合

GitHub
DropletUtils

液滴数据质控与空滴检测

Bioconductor
Scanpy

Python单细胞分析全流程

官方网站
Seurat

R语言单细胞分析标准工具包

官方网站
Scrublet

双细胞检测与过滤

GitHub
harmonypy

Python版Harmony批次校正

GitHub
harmonyR

R语言版Harmony批次校正

GitHub
scArches

单细胞参考图谱映射与迁移学习

GitHub
CellPhoneDB

细胞间通讯网络分析

GitHub
DESeq2

差异基因表达分析（伪bulk模式）

Bioconductor
Pegasus

大规模单细胞云端分析平台

官方网站
PAGA

细胞轨迹与分化路径推断

GitHub
CopyKAT

肿瘤细胞拷贝数变异检测

GitHub
cell2location

空间转录组细胞类型定位

GitHub

关键应用场景

  1. 数据预处理

  • CellRanger: 原始测序数据处理

  • DropletUtils: 液滴数据质控

  • Scrublet: 双细胞过滤

  • 数据整合

    • Harmony/harmonypy/harmonyR: 批次校正

    • scArches: 参考图谱整合

  • 核心分析

    • Scanpy/Pegasus: 标准化分析流程

    • Seurat: 双细胞过滤

    • DESeq2: 差异表达分析

    • PAGA: 发育轨迹重构

    • CopyKAT: 肿瘤异质性分析

  • 高级应用

    • CellPhoneDB: 细胞互作网络

    • cell2location: 空间位置解析

    统计方法

    Wilcoxon rank-sum test

    Wilcoxon signed-rank test

    a two-sided Bonferroni correction

    实验方法

    Immunohistochemistry (IHC) and neutral lipid staining

    Multiplexed Immunofluorescence

    Image analysis

    研究成果

    1.单细胞转录组学揭示非小细胞肺癌的异质性

    A 研究概述。

    B 队列概述。

    C UMAP投影和组合背景+健康数据集。

    D 用于肿瘤样本中广泛细胞类型注释的代表性基因的点图。

    E 等值线图显示了AT2细胞、CAML和AIMɸ中髓系和上皮基因的共表达。

    F 盒图显示AT2细胞、CAML和AIM中髓系和上皮基因的正常化、标度化和对数转化的基因表达。

    G 在CD235富集范围内计算的肿瘤和背景中非免疫细胞亚群的相对比例。

    2.整合的单细胞和空间转录组学揭示了LUAD和LUSC中不同的相互作用网络

    A 整合的单细胞和空间转录组学揭示了LUAD和LUSC中不同的相互作用网络。

    B 热图显示了LUAD和LUSC中广泛的细胞注释总结的所有细胞类型之间的LR相互作用数量。

    C ICI在LUAD和LUSC中的肿瘤特异性相互作用。

    D 突出显示的ICI基因和细胞类型的点图。

    E VEGFA/B相互作用物在LUAD和LUSC中的肿瘤特异性相互作用。

    F EGFR相互作用物在LUAD和LUSC中的肿瘤特异性相互作用。

    3.10x Visium证实了关键配体-受体对的空间共定位

    A 一张空间图像描绘了代表性肿瘤切片上AT2细胞、AIM和Tregs的细胞丰度。

    B 根据肿瘤和背景切片中细胞2位置的细胞丰度估计计算的免疫和非免疫细胞类型的相对比例。

    C 空间LR共定位热图。

    D 方框图显示了在所分析的每个切片中，肿瘤与背景中结肠定位LR对的频率存在显著差异。

    E 空间图像描绘了NRP1-VEGFA、NECTIN2-TIGIT、PD1-PDL1、CD96-NECTIN1和HAVCR2-LGALS9在肿瘤和背景中发现LR对共表达的斑点位置。

    4.CAML与肿瘤细胞共享肿瘤CNA和结肠

    A CNA分析(cancer-associated macrophage-like cells,CAML)。

    B PAGA图叠加在为肿瘤中的非免疫细胞类型计算的扩散图上。

    C 前三个面板指示肿瘤浸润区域、Visium斑点上肿瘤区域的分箱和通过QC的斑点。

    D 使用clusterProfiler R软件包对GO和REACTOME数据库进行过表示分析。

    E 患者的AT2和CAML中CNA的详细概述。

    F 肿瘤数据集中每种细胞类型之间损失和增益的KL散度散点图，使用其增益和损失分布计算。

    G 空间图像描绘了三个代表性肿瘤切片上AT2细胞和CAML的细胞丰度。

    H 在所有肿瘤切片中通过QC的斑点上，根据细胞类型组成计算的相关距离的分层聚类。

    I 非负矩阵因子化建立在q05对所有肿瘤切片中通过QC的点的细胞类型丰度的估计之上。

    5.肿瘤巨噬细胞经历癌胚重编程

    A 使用py_DESeq2包提取的肿瘤中AIMɸ的DEGs与背景的火山图。

    B GO注释M和AIM上调的DEGs。

    C 肿瘤和背景组织切片上CD68和中性脂质的IHC。

    D 肿瘤和背景切片中BODIPY信号覆盖的区域。

    E 肿瘤（左）和背景（右）组织切片上CD68和STAB1的IHC。

    F CD68+巨噬细胞群中STAB1+细胞的定量。

    G 肿瘤组织切片上CD68、STAB1和PanCK的染色。

    H NSCLC中CD68+巨噬细胞群内STAB1+CD68+细胞的定量。

    I 点图显示了肿瘤中所有巨噬细胞亚群和CAML中STAB1特征基因”的表达。

    J 肿瘤中肺泡Mφ与STAB1 Mφ的DEGs火山图。

    K GO富集分析。

    6.STAB1+Mɸ进行癌胚重编程

    A 对协调（肿瘤髓系+背景髓系+胎儿肺髓系）PC空间中每个细胞计算的相关距离进行分层聚类。

    B Violin图显示了在公开的人类胎儿肺图谱中鉴定的髓系细胞和祖细胞群体中STAB1基因特征的表达水平。

    C 选定胎儿肺巨噬细胞群中STAB1基因特征中每个基因表达的点图。

    D Violin图显示了在公开可用的MoMac VERSE数据集中鉴定的簇中STAB1基因特征的表达水平。

    E 来自MoMacVERSE的选定巨噬细胞群中STAB1基因特征”中每个基因表达的点图。

    F Violin图显示了在公开的“MoMac VERSE”数据集中鉴定的髓系细胞和祖细胞群体中AMɸ基因特征的表达水平。

    G Violin图显示了在公开的人类胎儿肺图谱中鉴定的髓系细胞和祖细胞群体中AMɸ基因特征的表达水平。

    H “MoMac VERSE”数据集中确定的选定巨噬细胞群体中AM基因特征中每个基因表达的点图

    I 选定胎儿肺巨噬细胞群中AMɸ基因特征中每个基因表达的点图。

    Reference

    Cui, X., Liu, S., Song, H. et al. Single-cell and spatial transcriptomic analyses revealing tumor microenvironment remodeling after neoadjuvant chemoimmunotherapy in non-small cell lung cancer. Mol Cancer 24, 111 (2025).

    肺组织相关细胞和markers

    Lung<-read.table("../Human_Lung.txt",sep="\t",header = T)
knitr::kable(Lung[,3:4])

    CellType

    Marker

    Activated CD4-positive, alpha-beta T cell

    CTLA4, IL2RA

    Adult endothelial progenitor cell

    C7, CCL4, CCL5, CLU, CXCL2, FCN3, HPGD, NKG7, POSTN

    Alpha-beta T cell

    CD3E, CD40LG, IL7R

    Alternatively activated macrophage

    CCL18, IL10, ADORA3, C1QA, C1QB, CD163, CD209, CD68, IL4I1, IRF4, MARCO, MMP12, MRC1, SOCS3, STAT6, WNT5A

    Alveolar capillary type 2 endothelial cell

    HPGD

    Alveolar macrophage

    CD68, FABP4, FOLR3, MARCO, APOC1, C1QA, C1QB, CBX5, FBP1, FGFR2, GSN, LYZ, MME, MRC1, MSR1, NMB, NUBP2, PPARG

    Alveolar monocyte

    ALOX5AP, APOC1, APOE, GPNMB, INHBA

    Anterior foregut endoderm cell

    CPM

    B cell

    CD79A, CD19, MS4A1, CD79B, IGHM, CD24, IGKC, MZB1, IGHG1, IGLL5, JCHAIN, BANK1, BLK, IGHA1, IGHG3, PAX5, SDC1, CD68, CD74, CR2, DERL3, FAM30A, FCRL2, FCRL5, IGHG4, IGLC3, IGLV3-1, PNOC, PTPRC, SPIB, TCL1A, TNFRSF13C, TNFRSF17

    Basal cell

    KRT5, TP63, KRT14, KRT17, KRT13, NGFR, DAPL1, IL33, S100A2, ITGA6, KRT15, KRT8, LAMB3, APOC1, APOE, BCAM, BMP7, CD44, CTNNB1, DST, EMP3, G0S2, ITGB, ITGB4, KRT6A, LAMA3, LAMC2, LGALS1, MIR205HG, MKI67, NPPC, PDPN, SOX2, SYT8, TOB1, VIM

    Basophil

    CPA3, MS4A2, TPSAB1, CCR5, SMAD1

    Brush cell

    ASCL2, DCLK1, ALOX5AP, GNG13, IRAG2, POU2F3, RGS13, TRPM5

    Cancer-associated fibroblast

    COL1A1, COL1A2, FAP, THY1, COL6A1, COL6A2, COL6A3, DCN

    Capillary endothelial cell

    CA4, CD36, PRX, RGCC, SGK1, SPARC

    CD14-low, CD16-positive monocyte

    FCGR3A, MS4A7

    CD14-positive monocyte

    S100A8, CD14, CPVL, LYZ, S100A12, S100A9, VCAN

    CD16-negative natural killer cell

    CD160, GNLY, NKG7

    CD16-positive natural killer cell

    FCGR3A, GNLY, NKG7

    CD4-intermediate, CD8-positive double-positive thymocyte

    CCR9, SATB1, TOX2

    CD4-positive, alpha-beta T cell

    CD3E, CD8A, CD8B, COTL1, LDHB, CD4, CD3D

    CD8-positive, alpha-beta cytotoxic T cell

    CD3D, CD8A, GNLY, CD8B, GZMB, PRF1

    CD8-positive, alpha-beta exhausted T cell

    HAVCR2, CXCL13, ENTPD1, LAG3

    CD8-positive, alpha-beta T cell

    CD8A, CD3E, DUSP2, GZMK, CD8B, MKI67, CCL5, CCNA2, CD3D, GZMA, GZMB, ITGAE, NKG7, NUSAP1

    Central memory CD4-positive, alpha-beta T cell

    CCR7, CD4, SELL

    Central memory CD8-positive, alpha-beta T cell

    CCR7, CD8A, SELL

    Central memory T cell

    CCR7, IL7R, SELL

    Cholangiocyte

    EPCAM, KRT19, KRT7

    Ciliated cell

    FOXJ1, CAPS, CCDC78, TPPP3, TP73, TUBB1, C20orf85, CETN2, EPCAM, MUC5AC, PIFO, RFX2, TEKT1, TMEM190, TUBA1A

    Ciliated epithelial cell

    CAPS, CDHR3, CIBAR2, DNAH5, DNAI1, DNAI2, FOXJ1, HYDIN, RFX2, TPPP3, TUBA1A

    Classical monocyte

    CD14, S100A8, S100A12, S100A9

    Club cell

    SCGB1A1, SCGB3A2, SCGB3A1, CCKAR, MUC5B, BPIFB1, BPIFA1, CP, GABRP, PIGR, SLC23A2, SLPI, TMEM45A, TSPAN8

    Dendritic cell

    CD1C, CCL17, CD1A, CD207, CD83, CLEC9A, IL3RA, ITGAE, ITGAX, LILRA4, CCL22, CD209, CLEC4C, HLA-DQA1, HLA-DQA2, HLA-DQB1, HLA-DQB2, THBD, CCL13, CCR6, CCR7, CD1E, CD68, CLEC10A, CYP2S1, FCER1A, HLA-DPA1, HLA-DPB1, HLA-DRA, HLA-DRB1, HSD11B1, IRF7, ITGAM, JCHAIN, MHC Class II, NDRG2, PACSIN1, PCX3CR1, PFLT3, PKIB, PTPRC, SEC11C, TCF4

    Dendritic cell, human

    CD1C, CLEC10A, FCER1A

    Deuterosomal cell

    CDC20B, DEUP1, FOXJ1, FOXN4

    Early alveolar monocyte

    GPNMB, IL7R, INHBA

    Effector CD8-positive, alpha-beta T cell

    CCL5, GZMB

    Effector memory CD4-positive, alpha-beta T cell

    AQP3, ITGB1, KLRB1

    Effector memory CD8-positive, alpha-beta T cell

    GZMK, CCL5, CD8A, CXCR6, GZMA, ITGA1, ITGAE

    Effector memory CD8-positive, alpha-beta T cell, terminally differentiated

    CX3CR1, GNLY, GZMB

    Effector memory T cell

    DUSP4, GZMA, GZMK, IFNG, PDCD1

    Effector T cell

    CX3CR1, FCGR3A, FGFBP2

    Endothelial cell

    PECAM1, CLDN5, VWF, CDH5, CD34, FLT1, EMCN, ESAM, KDR, MCAM, PLVAP, RAMP2, ABI3BP, ACKR1, ANXA3, AQP1, CAV1, CDC25B, DONSON, EPAS1, ERG, ESM1, HMGA1, INMT, LYVE1, NAPSA, NDRG4, NPR3, PAPSS2, RNF144B, SDC4, SELE, SELP, SEMA3F, SFTPA1, SFTPA2, SFTPB, SFTPC, SLCO4A1, SLPI, TMEM100

    Endothelial cell of artery

    GJA5, BMX, FBLN5, EFNB2, GJA4, HEY1, LTBP4, SEMA3G, SOX17

    Endothelial cell of lymphatic vessel

    PROX1, CCL21, LYVE1, PDPN, FLT4, ACTA2, CNN1, MMRN1, RELN, RGS5, TAGLN, VWF

    Endothelial cell of vascular tree

    BACE2, CD300LG, CLDN5, EPHB4, LDB2, NOSTRIN, PECAM1, PTPRB, RAMP3, VWF

    Endothelial cell-endothelial to mesenchymal transition

    AQP1, CAV1, DCN, EPAS1, FN1, LUM, SPARC

    Eosinophil

    SIGLEC8, RNASE3

    Epithelial cell

    CDH1, EPCAM, KRT19, KRT18, KRT8, CAPS, CLDN4, KRT5, KRT7, MUC1, SFTPB, FOLR1, FXYD3, KRT14, KRT15, KRT17, KRT6A, KRT78, KRT80, SEC61G, SFTA2, SFTA3, SNTN, UPK3B, AGER, ANPEP, CAV1, CD1D, CD24, CLDN5, CLDN6, COL4A1, EMP2, FLT1, FOXJ1, IL6R, IL6ST, ITGAE, KRT1, KRT10, KRT12, KRT13, KRT16, KRT2, KRT20, KRT23, KRT24, KRT25, KRT26, KRT27, KRT28, KRT3, KRT31, KRT32, KRT33A, KRT33B, KRT34, KRT35, KRT36, KRT37, KRT38, KRT39, KRT4, KRT40, KRT6B, KRT6C, KRT71, KRT72, KRT73, KRT74, KRT75, KRT76, KRT77, KRT79, KRT81, KRT82, KRT83, KRT84, KRT85, KRT86, KRT9, MUC16, MUC5AC, NOS2, OSMR, PIFO, RAMP2, S100A2, SCGB1A1, SDC1, SFN, SFTPA1, SFTPC, SLPI, THBD, TMEM190, TP63, VCAM1

    Epithelial fate stem cell

    LGR6

    Epithelial progenitor cell

    CEACAM6

    Erythrocyte

    HBB, HBA1, HBA2, ALAS2, DNASE1L3, SLC25A37

    Exhausted T cell

    LAG3, CXCL13, HAVCR2, LAYN, PDCD1, PRDM1, TIGIT

    Fat cell

    ACSL1, ADIPOQ, EBF1, FOXO1, G0S2, GHR, LPIN1, PLIN1, PLIN2, PPARG

    Fibroblast

    COL1A1, DCN, PDGFRA, COL1A2, THY1, BGN, C1R, LUM, APOE, PLIN2, ACTA2, ACTG2, CFD, COL10A1, COL11A1, COL14A1, COL3A1, COL4A1, DPT, FABP5, FAP, FBLN1, ITGA8, PDGFRB, PTGDS, TCF21, VIM, A2M, ADGRE5, ANGPT1, APOD, ASPN, CCN2, CD36, COL18A1, COL5A1, COL5A2, COL6A1, COL6A2, COL6A3, COL6A6, COX4I2, CTSK, DDR2, DES, FABP4, FBN1, FLNB, FN1, GJA4, GSN, HGF, HIGD1B, HTRA3, IGFBP3, IGFBP5, IL33, IL6, LIPA, LPL, MCAM, MFAP5, MGP, MMP11, MMP2, MT2A, MYL9, MYLK, NDUFA4L2, NEGR1, NOTCH3, NXF1, OSMR, PDGFRL, PDPN, PI16, PPP1R14A, PTN, PTP4A3, PTPN13, RGS5, SEC14L2, SFRP4, SLIT2, SULF1, USO1, VCAN, VEGFD

    Follicular B cell

    CD40, CD69, MS4A1

    Glial cell

    GFRA3

    Goblet cell

    MUC5AC, MUC5B, SPDEF, GABRP, LIPF, SCGB1A1, TFF1, TFF2

    Granulocyte

    CEACAM8, S100A12

    Group 3 innate lymphoid cell, human

    IL4I1, KIT, RORC

    Helper T cell

    CD4, CXCL13, GP1BA

    Hepatocyte

    ALB, HPR, MASP2

    Hillock cell

    KRT13, KRT4

    Inflammatory macrophage

    CXCL10, CALHM6, CD68, CXCL11, CXCL12, CXCL9, HLA-DR family, STAT1

    Intermediate monocyte

    CD14, FCGR3A, FCGR3B, S100A8

    Kupffer cell

    LYZ, MARCO, S100A8, S100A9

    Langerhans cell

    CD1A, CD1C, CD1E, FCER1A

    Late alveolar monocyte

    APOC1, APOE, INHBA

    Lung ciliated cell

    FOXJ1, CAPS, C20orf85, CDHR3, PIFO, TMEM190, ALDH3B1, ATP5IF1, CCDC78, CFAP126, DNAAF3, DNAH11, DNAH12, DNAH5, DNMBP, EPCAM, FAM183A, FOXK1, HOATZ, LRRIQ1, SNTN, TPPP3, TSPAN1, TUBB4B

    Lung microvascular endothelial cell

    CCL21, CDH5, PECAM1, VWF

    Lung neuroendocrine cell

    ASCL1, CHGA, CALCA, S100A12, HOXB5, INSM1, KRT8, SYP, UCHL1

    Lymphatic endothelial progenitor cell

    CCL21, FABP4, TFF3

    Macrophage

    CD68, CD14, MARCO, CD163, MSR1, CSF1R, MRC1, APOE, C1QA, FABP4, ITGAM, ITGAX, SPP1, C1QB, FCGR1A, FCGR3A, FCN1, FOLR2, CCL18, CCL2, CCR2, CD74, CD80, CD86, CX3CR1, LYZ, MCEMP1, MS4A4A, PCLAF, PTPRC, RNASE1, TREM2, CBX7, CCL13, CCL3L1, CD36, CD5L, CD69, CD83, CD84, CEBPB, CHI3L1, CPM, CRIP1, F13A1, FCER1G, FCGR3B, GLDN, HAMP, HLA class II, ICAM1, IL1RN, ITGAL, LGMN, LYVE1, MERTK, MMP9, MR1, MS4A6A, NUPR1, OSM, PLA2G7, RBPJ, RETNLB, RPS23, RPS24, RPS27, SELENOP, SLC11A1, SLC40A1, TFRC, UBB

    Marginal zone B cell of lymph node

    IGLL5

    Mast cell

    TPSAB1, CPA3, TPSB2, MS4A2, KIT, HDC, GATA2, HPGDS, CLU, PTGS1, RGS13, CD68, CD69, CMA1, CSF3R, CXCL8, CXCR2, ENPP3, FCER1A, FCGR3B, FFAR2, G0S2, PTPRC, S100A8

    Mature NK T cell

    CD3E, CD8A, FCER1G, TYROBP, CD3D, NCR3

    Megakaryocyte

    PF4, PPBP, NRGN, OST4, MDK, MVK

    Melanocyte

    MLANA, PMEL

    Memory B cell

    CD27, CR2, MS4A1, CD1C, CD24, CXCR3, FAS, IRF8

    Memory T cell

    CD69, CXCR6, IL7R, ITGAE, LTB, MYADM, PTPRC

    Mesenchymal cell

    PDGFRA, PDGFRB, VIM, COL1A2, COL3A1, DCN, POSTN, S100A4, SNAI1, SNAI2, TCF21, ZEB1, ZEB2

    Mesothelial cell

    MSLN, UPK3B, WT1, CDH2, KRT19

    Migratory DC

    CCL22, CCR7, FSCN1, LAMP3

    Migratory dendritic cell

    CCL17, CCL19, CCR7, BIRC2, BIRC3, CCL22, CD200, CD274, CD80, ENO3, FAS, FSCN1, IDO1, IL12B, IL4I1, LAMP3, MARCKS, PDCD1LG2, RELB, SOCS2, TRAF1

    Monocyte

    CD14, FCN1, CD68, FCGR3A, APOE, CCR2, CD163, CSF1R, CX3CR1, FCGR1A, ITGAM, ITGAX, MARCO, MCEMP1, MRC1, MSR1, S100A8, S100A9, LYZ, VCAN, CAMP, CCL2, CD24, CD300E, CD36, CD74, EREG, FN1, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5, JARID2, LDHB, LGALS2, S100A12, STMN1

    MregDC

    CCL17, CCL22, CCR7, LAMP3

    Myelocyte

    CSTA, FTL, S100A8

    Myeloid dendritic cell

    CD1C, CD74, HLA-DMA, HLA-DMB, HLA-DOA, HLA-DOB, HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQA2, HLA-DQB1, HLA-DQB2, HLA-DQB3, HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5, CLEC9A, LAMP3, PLD4, ADGRE1, CCR7, CD1A, CD1B, CD207, CD209, CD4, CD40, CD80, CD83, CD86, FCER1A, HLA-DR family, ITGA4, ITGAM, ITGAX, LY75, MHC Class II, NRP1, PDCD1LG2

    Myofibroblast cell

    ACTA2, COL1A1, PDGFRA, ELN, MYH11, PDGFRB, TAGLN, ACTG2, Calponin family, DES, FN1, LUM, MYL9, MYLK, PLN, RGS5, SPINT2

    Naive B cell

    CD69, CD80, CD86, FCER2

    Naive T cell

    PTPRC

    Naive thymus-derived CD4-positive, alpha-beta T cell

    CCR7, CD4, CD3E, LEF1, C1orf162, SELL, GPR183

    Naive thymus-derived CD8-positive, alpha-beta T cell

    CD8A, CD3E, CD8B, GZMB, GZMH, CCR7

    Natural killer cell

    NKG7, KLRD1, GNLY, KLRB1, KLRC1, NCR1, NCAM1, TYROBP, XCL2, CD3D, FGFBP2, KIR2DL3, SEC11C, XCL1, ASCL1, CALCA, CD160, CD8A, CX3CR1, FCGR3A, GZMA, GZMB, GZMH, IFNG, IL32, KLRA1P, KLRF1, NCR3, PRF1, UCHL1

    Neuroendocrine cell

    ASCL1, CALCA, CHGA

    Neuron

    ASCL1, CADM1, ELAVL4, GRP, MPZ, NNAT, S100B, STMN2, SYNGR1, TUBB2B

    Neutrophil

    FCGR3B, S100A8, S100A9, CSF3R, CXCR2, IFITM2, ADGRG3, ALPL, CEACAM3, CEACAM8, CMTM2, CXCL8, CXCR1, FCAR, FFAR2, FPR1, G0S2, ITGAM, MME, MMP25, PROK2, S100A12, SIGLEC5, TNFRSF10C

    Non-classical monocyte

    FCGR3A, FCGR3B, C1QA, CX3CR1

    Parasympathetic neuron

    SNAP25

    Pericyte

    PDGFRB, CSPG4, TRPC6, RGS5, ACTA2, ANPEP, COX4I2, DES, GUCY1B1, HIGD1B, KCNK3, MCAM, MYO1B

    Plasma cell

    JCHAIN, CD79A, IGKC, CD27, IGHG4, SLAMF7, IGHA1, IGHG1, CD68, CD9, IGHA2, IGHG2, IGHG3, IGLC2, MZB1, PTPRC, SDC1, TNFRSF17, XBP1

    Plasmablast

    XBP1, IRF4, JCHAIN, MZB1, PRDM1

    Plasmacytoid dendritic cell

    LILRA4, CLEC4C, IRF8, LILRB4, IL3RA, IRF7, LRRC26, SCT, CXCR3, DNASE1L3, GZMB, IDO1, IRF4, JCHAIN, LAMP5, MZB1, NRP1, PACSIN1, SHD, SLC32A1, TCF4, TCL1A, THBD, TLR7

    Plasmacytoid dendritic cell, human

    IL3RA, LILRA4, PLD4

    Pneumocyte

    CLDN18, SFTPA1, SFTPA2, SFTPC, AGER, AQP4, NAPSA, ACTA2, CAV1, COL1A1, COL3A1, CPM, CXCL14, DCN, DES, EMP2, FOLR1, LAMP3, MMP7, MYL9, MYLK, NKX2-1, PDGFRA, PEBP4, PGC, SFRP4, SFTPB, SFTPD

    Pre-alveolar type-1 transitional cell

    CLDN4, KRT8, LGALS1, LGALS3, SFN

    Progenitor cell

    TM4SF1, AGER, ATXN1, CAV1, CD34, MUC5B, NKX2-1, RTKN2, SCGB3A2

    Pulmonary ionocyte

    ASCL3, CFTR, FOXI1, FXYD6, ID3, JADE1, RARRES2, TMEM160, TPM1

    Regulatory T cell

    FOXP3, IL2RA, CTLA4, TNFRSF18, TNFRSF4, CCR7, CCR8, CD27, CD3E, CD40LG, IKZF4, TNFRSF9

    Smooth muscle cell

    ACTA2, TAGLN, DES, MYH11, CNN1, LGR6, MYL9, ACTG2, ACTN1, COL1A1, COL3A1, GUCY1A1, GUCY1B1, MMP11, MYL6, MYLK, MYOCD, OSMR, PALLD, PDGFRB, SMTN, VCL

    Stratified epithelial cell

    KRT19, KRT7, PIGR, WFDC2

    Stromal cell

    ACTA2, DCN, LUM, MGP, PDGFRB, PECAM1, VWF

    Suprabasal cell

    KRT5, KRT19, NOTCH3, TP63

    T cell

    CD3D, CD3E, CD3G, CD8A, TRAC, CD2, TRBC1, NKG7, TRBC2, CD28, CD4, CD69, CD8B, CCL5, CD27, FOXP3, GZMA, GZMB, GZMK, IL7R, LTB, PTPRC, CD247, CD6, CD68, CD96, CTLA4, GNG4, GNLY, IL2RA, IL32, KLRD1, MKI67, NCAM1, PDCD1, SH2D1A, SKAP1, THEMIS, TOP2A, TRAT1, ZNF683

    T follicular helper cell

    CXCR5, ICOS, PDCD1

    T-helper 1 cell

    TBX21, CCL5, CXCR3, IFNG, TNF

    T-helper 17 cell

    CCR6, IL7R, ZBTB16

    Tip cell

    ESM1, NID2

    Tissue-resident macrophage

    CCL4, CXCL10, CXCL11, FABP4, ISG15, ISG20

    Type 1 conventional dendritic cell

    CLEC9A, XCR1, BATF3, BTLA, CADM1, CCL19, CCL22, CCR7, CD1C, EBI3, IDO1, LGALS2, NDRG2

    Type 2 conventional dendritic cell

    CD1C, CD1E, CLEC10A, FCER1A, CD1A, NLRP3, SIRPA

    Type I pneumocyte

    AGER, PDPN, CAV1, HOPX, AQP5, CLIC5, EMP2, CDH1, KRT19, SFTPC, ABCA3, AQP4, EPCAM, GPRC5A, KRT7, RTKN2, SFTPB, SLPI, TJP1

    Type II pneumocyte

    SFTPC, SFTPB, SFTPA1, SFTPD, SFTPA2, LAMP3, MUC1, PGC, SLC34A2, NAPSA, SFTPA3, ABCA3, ETV5, EPCAM, LPCAT1, MDK, NKX2-1, AGER, ALPG, CD44, HHIP, SLPI, WFDC2

    Vascular associated smooth muscle cell

    ACTA2, CNN1, RGS5, TAGLN, LGR5, NOTCH3, PDGFRB

    Vein endothelial cell

    ACKR1, SELP, COL15A1, NR2F2, VCAM1, VWA1

网站公告

今日签到

点亮在社区的每一天
去签到

热门文章

最新发布