Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA.
The antibody reacted with mouse IgG whole molecule. It also reacts with the light chains of all other types of immunoglobulins in mice. After special treatment, its non-specific binding to human, rabbit, goat, sheep and guinea pig serum proteins was reduced to the minimum. It has no reactivity to non immunoglobulin serum proteins.
Abbkine donkey anti mouse IgG secondary antibody, green fluorescent labeling basic information:
English name IFKine ™ Green Donkey Anti-Mouse IgG
Immunogen Mouse IgG
Host donkey
Reactive mice
Application FCM, ICC, IF
The experimental suggestions need to be optimized. For most immunofluorescence experiments, the recommended initial dilution ratio is 1:50-1:1000.
Clonal polyclonal
Purification process Affinity purification: The solid phase coupled mouse immunoglobulin IgG (H+L) light chain was used, and the protein purity was up to>95% (SDS-PAGE detection).
Form Liquid solution
Storage buffer PBS buffer (pH 7.4), containing 0.02% sodium azide (preservative, 1% BSA (as protein stabilizer) and 50% glycerol.
It is recommended to keep it stable for 1 year at - 20 ° C. To avoid loss, melt the antibody and centrifuge before opening the tube cap. It is recommended to repack before use to avoid repeated freezing and thawing.
Transportation conditions Blue ice transportation.
Abbkine IFKine驴抗小鼠IgG二抗，绿色荧光中文说明书：
背景：
Abbkine二级抗体可与酶、生物素或荧光团结合，用于多种基于抗体的应用，包括蛋白质印迹、免疫组织化学、免疫荧光、流式细胞术和ELISA。
Abbkine驴抗小鼠IgG二抗，绿色荧光标记抗体与小鼠IgG全分子反应。它也与小鼠所有其它类型的免疫球蛋白的轻链发生反应。经过特殊处理，它对人，兔，山羊，绵羊，豚鼠血清蛋白的非特异性结合降到了最低。它对非免疫球蛋白的血清蛋白没有反应性。
Abbkine IFKine驴抗小鼠IgG二抗，绿色荧光实验建议：
需要摸索优化。对于大多数免疫荧光实验，建议的起始稀释比例为：1:50-1:1000。
Abbkine驴抗小鼠IgG二抗，绿色荧光标记特点：
IFKine™是一系列独特荧光染色二抗产品，具有更强的亮度，光稳定性和更低的非特异性杂交和背景。新一代的IFKine 荧光染料基团确保了J佳的荧光性能，驴来源和经过其它物种血清吸附的特点，使IFKine荧光二抗成为荧光染色的理想选择，尤其是在荧光多标实验。